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Revista mexicamãng cầu de fitopatología

versão On-line ISSN 2007-8080versão impressa ISSN 0185-3309

Rev. mex. fitopatol vol.36 no.3 Texcoco Out./Dez. 2018

Early morphological development of sclerotia of Sclerotinia sclerotiorum in the presence of potassium bicarbonate

Claudia Ordóñez-Valencia1 

Ronald Ferrera-Cerrato1 

Alejandro Alarcón1 * 

Laura V. Hernández-Cuevas2 

John Larsen3 

1 Área de Microbiología, Posgrado de Edafología.Colegio de Postgraduados. Carretera México-Texcoteo Km 36.5. Montecillo, Texcoco,Estavày de México, CPhường. 56230 México

2 Centro de Investigación en Genética yAmbiente. Universidad Autónoma de Tlaxcala. Autopista Texmelucan-Tlaxcala Km10.5. Ixtacuixtla CPhường. 90120, Tlaxcala, México

3 Institukhổng lồ de Investigaciones en Ecosistemas ySustentabilidad, Universidad Nacional Autónoma de Méxiteo. Antigua Carretera aPátzcuaro No.8701, Colonia Ex Hacienda de San José de la Huerta, Morelia,Michoacán, CPhường. 58190 Méxiteo.

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Sclerotinia sclerotiorum is a pathoren of great economic importance that causes significant losses in various crops. Control of the pathogene is difficult since this fungus forms resistant sclerotia that can survive in the soil for many years. This study evaluated the morphological development of S. sclerotiorum sclerotium primordia by using the Riddell technique, & different concentrations of potassium bicarbonate (KHCO3). The formation of primordia began from hyphae. However, as the concentration of KHCO3 increased, morphological changes were observed in the initiation phase of the sclerotia, as well as in the inhibition of their development when using a 50 mM concentration of KHCO3. This chemical compound modifies the morphology và inhibits the development of sclerotia in their initial stages; hence it may offer potential as an alternative sầu khổng lồ synthetic fungicides for the control of plant diseases caused by S. sclerotiorum.

Key words: antifungal agent; morphogenesis; inhibition; microscopy


Sclerotinia sclerotiorum es un patógeno de suma importancia económica quecausa grandes pérdidas en varquả táo cultivos. Controlar este patógeno es difícilporque forma estructuras de resistencia llamadas esclerocgame ios que puedenmantenerse viables en el suelo por muchos años. Este estudio evaluó eldesarrollo morfológico de los primordios de escleroctiện ích ios de S.sclerotiorum utilizanvị la técnica de Riddell, y diferentesconcentraciones de bicarbonato lớn de potasio (KHCO3). La formación delos primordios de los escleroctiện ích ios inició a partir de hifas; sin embargo,conforme las concentraciones de KHCO3 incrementaron, se observaroncambquả táo morfológicos en la fase de iniciación de los escleroctiện ích ios, así como en lainhibición de su desarrollo al utilizar una concentración de 50 mM deKHCO3. Este compuesto químico modifica la morfología e inhibe eldesarrollo de esclerocios en su fase inicial y, por tanto, podría utilizarsecomo alternativa a los fungicidas sintéticos para controlar enfermedades deplantas causadas por S. sclerotiorum.

Palabras clave: agente antifúngico; morfogénesis; inhibición; microscopia

White mold is caused by Sclerotinia sclerotiorum, a fungus that belongs to lớn the family Sclerotiniaceae. This is a destructive fungal pathogene for many agricultural crops such as sunflower, soybean, oilseed rape, bean, chickpea, canola, và onion (Hegedus và Rimmer, 2005: Bolton et al; 2006). It has little host specifithành phố, thus being able khổng lồ infect over 400 plant species, mainly dicotyledons (Fernanbởi et al., 2004; Hegedus & Rimmer, 2005). The environmental conditions that promote the fungal infection are high humidity and temperatures between 15 & 25 °C (Saharan and Mehta, 2008). Secretion of fungal enzymes such as cellulases and pectinases, that soften và degrade plant tissues are involved in the plant infection process (Fernanbởi vì et al., 2004; Bolton et al., 2006), as well as production of oxalic acid, which has toxic effects on the tissue of the host (Hegedus and Rimmer, 2005). One characteristic of this pathoren is the formation of sclerotia, fungal structures of resistance and dispersal, which under favorable conditions can remain viable for several years in soils (Bae và Knudsen, 2007; Calvo và Cary 2015; Smith et al., 2015).

During the formation of sclerotia, three stages or phases have sầu been identified: 1) initiation: aggregation of hyphae, 2) development: hyphal growth for greater size, và 3) maturation: creation of surface boundaries, internal consolidation & melanization (Le Tourneau, 1979; Rollins và Dickman, 2001; Bolton et al., 2006; Saharan và Mehta, 2008). The initiation and maturation stages may be influenced by abiotic factors such as photoperiod, temperature, oxygen, and nutrient availability (e.g. carbon sources), và the morphogenesis và further development of sclerotia started between 12 và 24 h of fungal growth (Hansberg & Aguirre, 1990).

The sclerotium is composed by three layers: a thiông chồng & pigmented outer layer, an intermediate và thin layer, và an internal Trắng layer called the inner medulla (Punja và Damiani, 1996; Bardin and Huang, 2001). Depending on environmental conditions, sclerotia grow belowground in one of two ways: 1) by forming mycelium that potentially infects roots and causes rot và wilting of plant tissues, or 2) by producing apothecia, in which ascospores are produced and released, then infecting aerial plant mni-alive.comans (Humpherson-Jones & Cooke, 1977; Mónateo et al., 1998; Bolton et al., 2006).

Bicarbonates possess antimicrobial properties of wide spectrum, & their efficiency has been proven for controlling many plant pathogenic fungi (Bombelli và Wright, 2006; Arslan, 2015). The Environmental Protection Agency (EPA) of the United States also recognizes bicarbonates as innocuous & safe compounds for both human health và environment (Palmer et al., 1997; Bombelli và Wright, 2006), since their use may decrease the utilization of pesticides. Some studies have sầu shown that sodium, potassium và ammonium carbonates & bicarbonates inhibit the post-harvest growth of several fungal pathogens in fruits, vegetables và ornamental plants (Karabulut et al., 2003; Arslan et al., 2006; Jabnoun-Khiareddine et al., 2016). Bicarbonates alter the permeability of fungal membranes, inhibit the reactions of oxidative sầu phosphorylation, and exert toxic effects on the structures of the pathogene (Avis, 2007). However, their efficacy depends on the concentration (0.2-3%) và on the susceptibility of each micromni-alive.comanism. For instance, treatments with sodium carbonate & bicarbonate improved the control of the green mold caused by Penicillium digitatum Sacc. (Trichocomaceae), in citrus fruits (Smilaniông chồng et al., 1999). Sodium và potassium bicarbonates also reduced powdery mildew caused by Leveillula taurica (Lév.) Arnaud (Erysiphaceae) in peppers (Fallik et al., 1997), and decreased the conidiogenesis by Helminthosporium solani Durieu y Mont. (Pleosporaceae) (Olivier et al, 1998). In addition, Bombelli & Wright (2006), and Türkkan et al. (2017) observed the growth inhibition of Botrytis cinerea Pers. Fr. (Sclerotiniaceae) when exposed to lớn different bicarbonates with in vitro cultures. Plant disease control in carrot, cucumber & cantaloupe fruits has been also reported due to lớn the application of bicarbonates (Aharoni et al., 1997; Bombelli & Wright, 2006).

Overall, the inhibitory effects of potassium bicarbonate (KHCO3) on the growth of S. sclerotiorum, as well as on the germination & formation of new sclerotia have sầu been described (Ordóñez-Valencia et al., 2009), but the effects of this chemical compound during early stages of sclerotia morphological development are not well understood. Thus, the ayên of this study was lớn evaluate the effects of different doses of potassium bicarbonate on the early stages & primordia development of sclerotia by S. sclerotiorum via microscopic observations.


In order khổng lồ maintain the humidity in the Petri dishes, 10 mL of 10% glycerol were added. All Petri dishes were kept at room temperature conditions (~trăng tròn °C) & an approximate photoperiod of 12 h. Every day, the fungal growth was monitored under optical microscope (Leica CME, U.S.A.). Once the PDA-disk was fully covered with the fungal mycelium (approximately seven days of incubation), glycerol was replaced with a 10% formaldehyde solution, which was kept for 2 h for permanently fixing the fungal structures.

Later, the microscopic slide was removed from the Petri dish khổng lồ prepare the fungal slides. The cover slip was carefully separated from the agar và placed on another clean slide on which a drop of the colorant cotton blue in lactophenol was added. The next step was to remove sầu the PDA-disk from the original microscopic slide on which the colorant was also added, & a clean cover slip was immediately placed on it. In this way, four fungal preparations were obtained from each concentration of KHCO3, including the control without bicarbonate. Once the excess of colorant was removed, the stained fungal preparations were sealed with colorless nail polish, and evaluated under light microscope. The microscopic evaluations consisted on identifying the growth of the sclerotial primordia in each concentration of KHCO3. For this, an optical microscope (OLYMPUS BX51, Japan) was utilized for taking microphotographs of the fungal structures under phase contrast microscopy. Chi-square “goodness of fit” tests were performed in order to lớn compare the effect of different concentrations of sodium bicarbonate on sclerotia formation for each one of the four structure phases. For it was used the VassarStats: Web Site for Statistical Computation (Lowry 2001-2018).

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The presented fungal structures are part of the process of sclerotia formation by S. sclerotiorum, và were microscopically observed before the initiation stage. The sclerotial formation in the control treatment (Figure 1, A-C) began with the proliferation of primary branching of main hyphae, thus, denoting the formation of the sclerotial primordia. As second structure, the hyphal branching became more profuse, and the presence of septa was observed in apical zones (Figure 1, D-F), while the third structure was characterized by presenting small hyphal clusters (Fig. 1, G-I). Finally, in the fourth structure, a massive cluster of hyphae was observed, in which some pigmented cells were visible (Figure 1, J-L). The initiation stage began with the union of several hyphal clusters, and this stage was macroscopically observed when a hyphal conglomerate starts growing on the surface of the culture medium.

Figure 1 Microscopic developmental structures observed during the sclerotial formation of Sclerotinia sclerotiorum after seven days of fungal growth, without KHCO3 (Control). First structure: branching of hyphae, indicated by arrows (A-C); second structure: profuse branching of hyphae (D-F); third structure: clustering of hyphae (G-I); fourth structure: massive sầu clustering of hyphae that build up the sclerotia in the initiation process (J-L). Microphotographs were taken in phase contrast microscopy at 40X magnification. Bars = 10 µm. 

Results showed that the application of KHCO3 had inhibitory effects on the morphology of the sclerotial primordia (Table 1). In fact results from the 50 mM dose were not included on statistical analysis due the complete absence of mycelial growth. Unlikely the treatment without bicarbonate (control) và 2 mM bicarbonate, treatments with all the others concentrations of this chemical compound showed only three developmental structures, in which we noticed that the primordia were irregularly shaped with the formation of loose cells. As the concentration of KHCO3 increased, primordia became smaller và less compact. The increase in the concentration of bicarbonate resulted in growth inhibition of both hyphae và sclerotia (Figure 2 and 3). However, at 2 mM, 4 mM and 6 mM concentrations of KHCO3 only scarce morphological changes were observed.

Table 1 Frequencies of sclerotia on each structure phase. The value on last column indicates the probability of not effects due lớn sodium bicarbonate exposition for each structure phase (đưa ra square “goodness fit”). 

Tratamientos Concentraciones de bicarbonato de sodio
Fase de la estructura Control 2 mM 4 mM 6 mM 8 mM 10 mM 25 mM Probabilidad
I 6 5 5 6 5 5 7 0.8606
II 5 5 5 5 5 5 0 0.2914
III 4 5 5 6 5 5 0

Figure 2 Microscopic developmental structures observed during the sclerotial formation of Sclerotinia sclerotiorum, after seven days of fungal growth. First (indicated by arrows), second, and third structure of sclerotial development in presence of KHCO3: (A-C) 2 mM, (D-F) 4 mM, and (G-I) 6 mM. Microphotographs were taken in phase contrast microscopy at 40X magnification. Bars = 10 µm. 

Figure 3 Microscopic developmental structures observed during the sclerotial formation of Sclerotinia sclerotiorum, after seven days of fungal growth. First (indicated by arrows), second and third structures of sclerotial development in presence of KHCO3: (A-C) 8 mM, and (D-F) 10 mM. For 25 mM a cellular dismni-alive.comanization of the primordium was observed (G-I), in which the second and third structures of sclerotial development were not observed. Microphotographs were taken in phase contrast microscopy at 40X magnification. Bars = 10 μm. 

The most notorious morphological changes were evident after the fungus was exposed lớn concentrations greater than 8 mM of KHCO3. At 10 mM, the presence of primordia was noticed although not very well developed. Despite this, it was possible khổng lồ observe the third structures of development (Figure 3, F), but it was not similar to the control by showing irregular formations of the sclerotium in which hyphal clusters were more loose (Figure 1, G-I). At 25 mM concentration, the formation of sclerotia primordia was scarce và dismni-alive.comanized (Figure 3, G-I), hence either the second or third structures of the sclerotium initiation could not be completed nor observed (Table 1). Finally, at the 50 mM concentration, no effects were noticed due khổng lồ the absence of fungal growth in this treatment.

In this study, we observed the formation process of sclerotia during their initiation phase in which the four stages of development were identified (Bolton et al., 2006; Saharan và Mehta, 2008). However, the sclerotial formation showed variations depending on the concentration of KHCO3. The inhibitory effect of bicarbonates on the growth of several species of phytopathogenic fungi, especially during postharvest, has been recorded before (Aharoni et al., 1997; Palmer et al., 1997; Bombelli & Wright, 2006; Jabnoun-Khiareddine et al., 2016), và Ordóñez-Valencia et al. (2009) demonstrated that KHCO3 inhibited the growth of S. sclerotiorum in in vitro cultures. The inhibitory effect of bicarbonate salts on fungi was probably due to reduced fungal cell turgor pressure, which resulted in collapse & shrinkage of hyphae (Türkkan et al., 2017).

The formation of sclerotial primordia by S. sclerotiorum was initiated by branching and clustering of hyphae (Figure 1), resulting in a mass of cells that eventually originate mature sclerotia. Similar effects were observed by Smits và Noguera (1988) in the formation of sclerotia of Macrophomina phaseolimãng cầu, which began from hyphal branching và entwinements, besides the increase in kích thước of the associate cells và the reduction in kích thước of the sclerotial mass. Townsover & Willets (1954) observed different development patterns (thickening, branching, and septation of main hyphae và their entwinement) in Rhizoctonia solani, Botrytis allii, B. cinerea, và Sclerotium cepivorum.

In the present study, KHCO3 resulted in microscopic morphological changes during early phases of sclerotia development. The increase of bicarbonate concentrations resulted in less profuse & loose hyphal branching, leading to the decrement & consequent absence of well-formed sclerotia (Table 1). Igwegbe et al. (1977) reported that the addition of 50 µg mL-1 of 6-metilpurine caused significant reduction in the sclerotia formation by S. rolfsii.

The addition of KHCO3 caused an increase in pH (from 6.5 khổng lồ 8.0) in the culture medium (Ordóñez-Valencia et al., 2009), which resulted in reductions of fungal growth. In this regard, Alexander (1977) mentioned that many fungi grow better under acidic conditions than alkaline, because an acidic environment is not conducive to the existence of either bacteria or actinomycetes, resulting in the monopoly of fungi for utilization of mni-alive.comanic substrates (Giri et al., 2005). On the other hand, it has been observed that both growth and development of sclerotia of S. sclerotiorum depkết thúc on the pH and the production of oxalic acid (Rollins và Dickman, 2001; Chen et al., 2004). Neutral or alkaline pH values inhibit the formation of sclerotia, and the production of oxalic acid helps reducing the alkaline pH of the medium, creating more favorable conditions for the development of sclerotia (Rollins and Dickman, 2001).

Although some reports have sầu described the negative sầu effects of bicarbonate on certain plant pathogenic fungi (Bombelli & Wright, 2006; Ordóñez-Valencia et al., 2009), yet the present study is one of the first reports describing inhibitory effects of KHCO3 on S. sclerotiorum during the initial phases of the sclerotia formation as well as on the morphology of sclerotial primordia.

The inhibitory effects of KHCO3 on fungal growth & development may in part be explained by affecting vital biochemical processes such as the biogenesis of either the fungal cell wall and/or the apical wall (Sentandreu et al., 1994; Sideri & Gemni-alive.comiou, 2000). Certain antimicrobial compounds cause oxidative sầu căng thẳng in fungi which may show morphological changes, impaired growth rate, & low nội dung of proteins và ATP (Harel et al., 2005; Marcet-Houben và Gabaldon, 2011). In this regard, the application of KHCO3 may tài khoản on the production of reactive sầu oxyren species (ROS) as a response of the găng tay generated by this salternative text, then, causing alterations on the morphology & development of S. sclerotiorum.

Bicarbonate ions cause alterations in oxidation and nitration reactions in biologicalsystems, regulate pH, và stimulate the production of either reactive sầu nitrogenspecies such as peroxynitrite (ONOO-) or superoxide (O2-)(Knorev et al.,2000; Arai et al.,2005; Lushchak etal., 2009). As a result of oxidative sầu bức xúc in combinationwith abiotic factors also have sầu negative effects on the sclerotia formation infilamentous fungi (Gemni-alive.comiou etal., 2006). Moreover, Sideri and Gemni-alive.comiou (2000) demonstrated that the production ofhydroren peroxide (H2O2) in S. rolfsii(Typhulaceae) exposed to different light and iron conditions; the highestproduction of H2O2 was recorded during early stages offungal growth. However, as sclerotia become mature the hydrogen peroxideproduction decreased. Nevertheless, further research is needed to elucidate theeffects of KHCO3 on either physiological, biochemical or molecularprocesses during fungal morphogenesis.

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